Detection of biosimilar erythropoietins using isoelectric focusing and SDS-PAGE in doping control
- Detection of biosimilar erythropoietins using isoelectric focusing and SDS-PAGE in doping control
- 신상미; 강민정; 유혜현; 권오승
- Issue Date
- 2010 KSBMB Annual meeting
- This study was intended to develop a detection method for recombinant EPO biosimilars by Isoelectric focusing and SDS-PAGE.
The developed isoelectic focusing method showed limit of detection of 7 ng/L.
SDS-PAGE method needs immunoprecipitation for concentration and the magnetic bead method gave better result than the immunoassay kit method.
This analysis for recombinant EPO was suitable for discrimination of EPO biosimilars from endogenous EPO.
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