Detection of biosimilar erythropoietins using isoelectric focusing and SDS-PAGE in doping control

Title
Detection of biosimilar erythropoietins using isoelectric focusing and SDS-PAGE in doping control
Authors
신상미강민정유혜현권오승
Issue Date
2010-05
Publisher
2010 KSBMB Annual meeting
Abstract
This study was intended to develop a detection method for recombinant EPO biosimilars by Isoelectric focusing and SDS-PAGE. The developed isoelectic focusing method showed limit of detection of 7 ng/L. SDS-PAGE method needs immunoprecipitation for concentration and the magnetic bead method gave better result than the immunoassay kit method. This analysis for recombinant EPO was suitable for discrimination of EPO biosimilars from endogenous EPO.
URI
http://pubs.kist.re.kr/handle/201004/41739
Appears in Collections:
KIST Publication > Conference Paper
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