Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis
- Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis
- 김영오; 이태호; 박태주; 박희수; 이상협
- aerophilic biofilm; ammonia oxidizing bacteria; FISH; Nitrification; PCR-DGGE
- Issue Date
- The Korean journal of chemical engineering
- VOL 26, NO 3, 685-690
- This study was conducted by combining fluorescence in situ hybridization (FISH) performed on 16S rRNA
and polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) with 16S CTOs primers to characterize
the nitrifying microbial communities in biofilm processes, which were tested to retrofit the S municipal wastewater
treatment plant in Busan, Korea. Four aerophilic biofilm reactors were operated with hydraulic retention times
of 2 to 8 h and biofilms were grown on ceramic media. The same low COD/ NH4
+-N ratio (100 mg/L of COD over
40 mg/L of NH4
+-N) with the S plant was used. The average relative population ratios of Nitrosomonas spp. to ammonia
oxidizing bacteria (AOB) as measured by specific FISH probes (%/Nso190) were 75.0%, 80.0%, 73.0% and 73.5%,
respectively, while those of Nitrosospira spp. to AOB were 21.0%, 14.7%, 24.6% and 24.1% after 180 days of operation.
The microbial composition of Nitrobacter spp. detected by using a Nit3 probe was below 10% in each reactor.
In contrast, Nitrospira genus detected with an Ntspa662 probe was around 20%. When CTOs primer was applied in
PCR-DGGE analysis to define the nitrifying bacteria, the bands of group B in the R-1 reactor with the highest hydraulic
retention time (HRT) had the strongest light intensity compared with two other reactors with lower HRTs after day
64. The bands of the groups were responsible for nitrification with the major dominant population in each reactor depending
on the change of ammonia removal rate. These results would directly lead to an understanding of the reactor
performance in relation to the ammonia removal, when conventional municipal wastewater treatment plants are retrofitted
or upgraded to biological nitrogen removal processes using biofilm.
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