Heterogeneity in tumor cell energetic metabolome at different cell cycle phases of human colon cancer cell lines
- Heterogeneity in tumor cell energetic metabolome at different cell cycle phases of human colon cancer cell lines
- Sasidhar Maddula; J. I. Baumbach
- glycolysis; glutaminolysis; tumor energetic metabolome; pyruvate; phosphoenolpyruvate; lactate; cell cycle phases; metabolomics; colon cancer
- Issue Date
- VOL 7, NO 4, 509-523
- In this present study, the efficacy of metabolomics
as a tool for tumor cell energetics for in vitro cell
cultures was demonstrated with full competence for the
first time by elucidating the anabolic and energy-yielding
segments of glycolysis and glutaminolysis, which constitute
a part of energy metabolism in tumor cells. By synchronizing
colon cancer cells SW480 and SW620 in
culture, the metabolome specific to cell cycle phases was
analyzed using nuclear magnetic resonance spectroscopy.
At the G1/S transition of the cell cycle (i.e. transition from
cell growth to duplication of genetic material), the majority
of the energy production was realized by glycolysis
through a high channeling of glucose carbons towards
lactate. During the late S phase, the majority of energy was
produced by glutaminolysis through a high channeling of
glutamine carbons towards lactate, while the glucose carbons
were channeled towards bio-synthetic pathways.
These results indicate that the metabolism of proliferating
cells is heterogeneous throughout the cell cycle and can be
better interpreted on the basis of different cell cycle phases.
These findings could be exploited for the development of a
tool for tumor diagnosis as well as for targeting tumors.
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