A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae Radix extract

Title
A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae Radix extract
Authors
누럴정혜진김동현유혜현
Keywords
Scutellariae Radix; HPLC; isocratic elution; quality control
Issue Date
2012-11
Publisher
Natural product research
Citation
VOL 26, NO 21, 1957-1962
Abstract
Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, has been widely used in Asian countries for the treatment of dermatitis, diarrhoea, inflammatory disease and hepatic disease. A simple, sensitive and precise reversed-phase liquid chromatographic method with isocratic elution was developed to simultaneously determine four bioactive compounds in Scutellariae Radix: baicalein, baicalin, wogonin and wogonoside. Chromatographic analysis was performed on a YMC Pack Pro C8 column (150 4.6mm2, 3mm), with a mobile phase of 0.1% formic acid : acetonitrile (70 : 30, v/v) at a flow rate of 1.0mLmin 1, and UV detection at 280 nm. Linear behaviour was observed over the investigated concentration range (0.25–10 mgmL 1) for all analytes, with a correlation coefficient of 40.997. The intra- and inter-day precisions were 58.07%, and accuracies were 92.3– 102.9%. This method was successfully applied for the analysis of marker compounds for the quality control of Scutellariae Radix extract.
URI
http://pubs.kist.re.kr/handle/201004/42041
ISSN
14786419
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KIST Publication > Article
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