A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae Radix extract
- A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae Radix extract
- 누럴; 정혜진; 김동현; 유혜현
- Scutellariae Radix; HPLC; isocratic elution; quality control
- Issue Date
- Natural product research
- VOL 26, NO 21, 1957-1962
- Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, has been
widely used in Asian countries for the treatment of dermatitis, diarrhoea,
inflammatory disease and hepatic disease. A simple, sensitive and precise
reversed-phase liquid chromatographic method with isocratic elution was
developed to simultaneously determine four bioactive compounds in
Scutellariae Radix: baicalein, baicalin, wogonin and wogonoside.
Chromatographic analysis was performed on a YMC Pack Pro C8 column
(150 4.6mm2, 3mm), with a mobile phase of 0.1% formic acid : acetonitrile
(70 : 30, v/v) at a flow rate of 1.0mLmin 1, and UV detection at 280 nm.
Linear behaviour was observed over the investigated concentration range
(0.25–10 mgmL 1) for all analytes, with a correlation coefficient of 40.997.
The intra- and inter-day precisions were 58.07%, and accuracies were 92.3–
102.9%. This method was successfully applied for the analysis of marker
compounds for the quality control of Scutellariae Radix extract.
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