Investigation of endogenous metabolic changes in the urine of pseudo germ-free rats using a metabolomic approach
- Investigation of endogenous metabolic changes in the urine of pseudo germ-free rats using a metabolomic approach
- 이수현; 안지혜; 박현미; 정병화
- Gut microbiota; Biomarker; Global metabolic profiling
- Issue Date
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- VOL 887-888, 8-18
- Gut microflora are recognized as an active metabolic compartment in whole body systems. Understanding
their impact on host physiology is an ongoing process, although many studies demonstrate that they
play significant roles in host life. To assess the impact of gut microflora on host physiology in normal or
close to normal conditions of the intestine, we prepared pseudo germ-free rats by antibiotic treatment,
and we investigated urinary metabolite profiles of pseudo germ-free rats using UPLC–QTOF-MS based
on metabolomics. The repeatability and stability of the analysis were evaluated using QC samples and
testmixes in both positive and negative ionization modes. When data sets were analyzed with OPLSDA,
25 metabolites related to the activities of gut microflora were identified. The changes of amino acid
metabolism, especially aromatic or sulfur amino acids, and alternations of bioactive nutrients, such as
isoflavonoid and riboflavin were observed in the pseudo germ-free rats. Among the sulfur amino acid
metabolites, the metabolites reflecting oxidative stress increased in the urine of pseudo germ-free animals,
which imply that the activities of intestinal microorganisms can affect the host redox homeostasis.
Altered isoflavonoid metabolism due to lack of gut bacteria may impact on steroid hormone metabolism
in the body, especially estrogen metabolism. These results indicate that the some essential metabolic
pathways are sensitive to the activities of gut microorganism and directly or indirectly affected by the
state of intestinal bacteria, thus gut microflora plays an important role in whole body physiology.
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