Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production
- Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production
- 전승현; 이진우; 김병찬; 이지은; 주진; 박현민; 이종호; 이상목; 이도훈; 김상용; 구윤모; 신채호; 김승욱; 현택환; 김중배
- meso-structured onion-like silica; enzyme immobilization; enzyme stabilization; biodiesel production
- Issue Date
- Chemistry of materials
- VOL 24, NO 5, 924-929
- Meso-structured onion-like silica (Meso-Onion-S) was synthesized
and used as a host of enzyme immobilization. Meso-Onion-S has a 200−
300 nm sized primary meso-structured onion building unit, and each onion unit
has highly curved mesopores of 10 nm diameter in a multishell structure.
Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a twostep
process of enzyme adsorption and subsequent enzyme cross-linking, which
effectively prevents the leaching of cross-linked enzyme aggregates from highly
curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S
significantly improved the enzyme stability as well as the enzyme loading. For
example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days,
while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking
showed a rapid inactivation due to rigorous enzyme leaching under shaking.
Stable NER-LP was successfully employed to produce biodiesels and fatty acid
methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NERLP
was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in
the form of NER-LP in the presence of organic solvents.
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