Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production

Title
Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production
Authors
전승현이진우김병찬이지은주진박현민이종호이상목이도훈김상용구윤모신채호김승욱현택환김중배
Keywords
meso-structured onion-like silica; enzyme immobilization; enzyme stabilization; biodiesel production
Issue Date
2012-03
Publisher
Chemistry of materials
Citation
VOL 24, NO 5, 924-929
Abstract
Meso-structured onion-like silica (Meso-Onion-S) was synthesized and used as a host of enzyme immobilization. Meso-Onion-S has a 200− 300 nm sized primary meso-structured onion building unit, and each onion unit has highly curved mesopores of 10 nm diameter in a multishell structure. Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a twostep process of enzyme adsorption and subsequent enzyme cross-linking, which effectively prevents the leaching of cross-linked enzyme aggregates from highly curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S significantly improved the enzyme stability as well as the enzyme loading. For example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking showed a rapid inactivation due to rigorous enzyme leaching under shaking. Stable NER-LP was successfully employed to produce biodiesels and fatty acid methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NERLP was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in the form of NER-LP in the presence of organic solvents.
URI
http://pubs.kist.re.kr/handle/201004/42153
ISSN
08974756
Appears in Collections:
KIST Publication > Article
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