The Butanol Production Enhancement in a Clostridium Strain by an Electrochemically Introduced Reducing Equivalent

Title
The Butanol Production Enhancement in a Clostridium Strain by an Electrochemically Introduced Reducing Equivalent
Authors
최옥경우한민엄영순
Issue Date
2012-04
Publisher
한국생물공학회
Abstract
The redox balance in microbial metabolism is an important factor to determine end-products in the microbial fermentation. We supplied reducing power to a Clostridium strain to manipulate the redox metabolism using a cathode as a bioelectrochemical technique. The cyclic voltamogram showed a redox peak at -160 mV v.s. Ag/AgCl in the Clostridium culture grown in P2 medium with glucose as a carbon source. Electrochemically active Clostridium sp. was cultivated in a cathode compartment at an applied potential of -160 mV vs. Ag/AgCl. The electric current was consumed by the Clostridium without any mediator and it was increased to 1.6 mA according to the microbial growth. Interestingly, butanol production (4 NADH consumed for 1 butanol) increased up to 2.4 times, whereas the production of butyrate and acetate from glucose decreased in this bioelectrochemical system. We measured the ratio of NADH/NAD+ within cells and found it was dependent on the current change. It was supposed that the cathode reduction of NAD+ induced more butanol production. This is the first report of controlling the cellular metabolism of the electroactive Clostridium given by an electrochemically introduced reducing equivalent.
URI
http://pubs.kist.re.kr/handle/201004/42213
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KIST Publication > Conference Paper
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