Simple and accurate quantitative analysis of seven prohibited threshold substances in human urine by liquid chromatography/tandem mass spectrometry in doping control

Title
Simple and accurate quantitative analysis of seven prohibited threshold substances in human urine by liquid chromatography/tandem mass spectrometry in doping control
Authors
이강미김호준정은숙유혜현권오승진창배김동현이재익
Issue Date
2011-08
Publisher
Rapid communications in mass spectrometry : RCM
Citation
VOL 25, NO 16, 2261-2267
Abstract
A simple and accurate liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the quantitative determination of ephedrine, pseudoephedrine, methylephedrine, cathine, salbutamol, morphine and epitestosterone in human urine. Urine samples were spiked with internal standard and diluted with acetonitrile. After centrifugation, the supernatants were directly analyzed by LC/MS/MS using the selected reaction monitoring (SRM) mode. The linearity, intra‐ and inter‐day precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were evaluated and the method was found to be accurate and reproducible for the quantitation of threshold substances. When the method was applied to the analysis of blind urine samples for the proficiency test, the results were close to the nominal concentrations, within 87.7–106.6% of nominal values, suggesting that the developed methods can be successfully applied to routine doping analyses.
URI
http://pubs.kist.re.kr/handle/201004/42261
ISSN
0951-4198
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KIST Publication > Article
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