A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay
- A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay
- 이혜진; 김병찬; 오민규; 김중배
- Aptamer; Thrombin; enzyme; precipitate coating
- Issue Date
- Chemical communications
- VOL 48, NO 48, 5971-5973
- Stable enzyme precipitate coatings (EPCs) with high enzyme
loading were employed to develop a sensitive and reliable detection
protocol, termed EPC-linked aptamer assay (EPC-LAA).
EPC-LAA achieved the limit of detection to be 0.5 ng per mL of
thrombin (13.5 pM), and maintained a reasonably stable detection
signal even after storing all the reagents at 40 ℃ for 66 days.
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