A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Title
A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay
Authors
이혜진김병찬오민규김중배
Keywords
Aptamer; Thrombin; enzyme; precipitate coating
Issue Date
2012-06
Publisher
Chemical communications
Citation
VOL 48, NO 48, 5971-5973
Abstract
Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 ℃ for 66 days.
URI
http://pubs.kist.re.kr/handle/201004/42405
ISSN
13597345
Appears in Collections:
KIST Publication > Article
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