In-situ extractive fermentation of Megasphaera elsdenii NCIMB 702410 for production of hexanoic acid from sucrose as a sole carbon source

Title
In-situ extractive fermentation of Megasphaera elsdenii NCIMB 702410 for production of hexanoic acid from sucrose as a sole carbon source
Authors
최기은전병승우한민상병인엄영순
Issue Date
2012-04
Publisher
2012년 한국생물공학회 춘계학술대회
Abstract
Megasphaera elsdenii NCIMB 702410 is a gram-negative strictly anaerobic bacterium isolated in the rumen of cattle and sheep. This strain is able to utilize sucrose to produce 4.53 g/L of hexanoic acid in a batch culture. However, high-titer of hexanoic acid is toxic to the cellular growth. Therefore, the objective of this study is to improve hexanoic acid production of Megasphaera elsdenii by using reactive extraction method. The extraction of hexanoic acid was performed with 10 % (v/v) Alamine 336 (Cognis) in oleyl alcohol (DHW) as a solvent and this method led the selective removal of hexanoic acid from the fermentation broth with high recovery rate. Compared to conventional fermentation, in situ extractive fermentation showed a 5.7-folds higher production of hexanoic acid (25.83 g/L) due to immediate extraction of hexanoic acid. In addition, this extraction method was applied to fed-batch fermentation, resulting that the titer of hexanoic acid increased up to about 70.04 g/L (15.5-folds higher). In conclusion, the efficient extraction process is crucial for fermentative production of hexanoic acid by Megasphaera elsdenii NCIMB 702410 using sucrose as a sole carbon.
URI
http://pubs.kist.re.kr/handle/201004/42422
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KIST Publication > Conference Paper
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