Optimization of in-situ extractive fermentation for hexanoic acid production
- Optimization of in-situ extractive fermentation for hexanoic acid production
- 최기은; 전병승; 우한민; 상병인; 엄영순
- Issue Date
- 2012 한국 수소 및 신에너지학회 춘계학술대회 논문집
- Megasphaera elsdenii NCIMB 702410 is an obligate anaerobic bacterium isolated from the rumen of cattle. This strain is able to produce up to 4.53 g/L of hexanoic acid in batch culture using sucrose as a carbon source. Hexanoic acid, a six-carbon straight-chain fatty acid, is valuable chemical and can be converted to alkane fuels. The objectives of this study were to remove hexanoic acid produced from M. elsdenii NCIMB 702410 and improve productivity of hexanoic acid efficiently. In order to remove hexanoic acid in medium selectively, biphasic extraction model was used by 10 % (v/v) Alamine 336 in Oleyl alcohol. When the extraction model was evaluated with pH control (6.0), 90% of hexanoic acid in medium was extracted inner 10 hours. Therefore, the model was applied to extractive fermentation (in-situ extraction). The process was operated for 30 days using fed-batch system and concentration of hexanoic acid increased up to about 70.04 g/L (15.5-folds higher).
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