Biomonitoring of urinary di(2-ethylhexyl) phthalate metabolites of infants and parents using isotope dilution GC-MS

Biomonitoring of urinary di(2-ethylhexyl) phthalate metabolites of infants and parents using isotope dilution GC-MS
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Phthalates, also known as phthalate esters, are phthalic acid esters that are commonly used as plasticizers in manufacturing plastics, mainly polyvinyl chloride (PVC), in order to improve their flexibility, transparency, durability and longevity. The most frequently and widely used phthalate plasticizers are: di(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate (DIDP) and diisononyl phthalate (DINP) and among the former plasticizers, DEHP is dominantly used due to its low price. The plastic products may consist of DEHP from 1 % up to 40 %. DEHP is one of the most important phthalates for being the diester of phthalic acid with an ethylhexyl branch chain attached to the second carbon in the main chain (chemical formula C6H4(C8H17COO)2). Present study was aimed to biomonitor DEHP and its metabolites: mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) and mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) in urinary samples of infants and parents those who reside throughout nation Korea and to investigate the correlation between them if any exists. Liquid-liquid extraction (LLE) was performed in order to simultaneously analyze all three analytes. Trimethylsilylation (TMS) derivatization was employed and derivatives were analyzed using isotope dilution gas chromatography-mass spectrometry (GC-MS) method. Qualitative analysis of each analyte was carried out with corresponding isotope labeled internal standard and method detection limit (MDL) ranged from 0.05 ng/mL to 0.2 ng/mL. Calibration was progressed with selected concentrations between 0.2 ng/mL and 100.0 ng/mL and R2 values ranged from 0.9992 to 0.9999.Analyzed results may be used to study and assess human health risk from phthalate exposures, especially among infants and parents, in Korea. Furthermore, data that was collected in nationwide scale and its following interpretations may be used to make international comparison of level of
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