Online High Performance Liquid Chromatography (HPLC)-ABTS+ Based Assay and HPLC-Electrospray Ionization Mass Spectrometry Analysis of Antioxidant Phenolic Compounds in Salsola komarovii

Title
Online High Performance Liquid Chromatography (HPLC)-ABTS+ Based Assay and HPLC-Electrospray Ionization Mass Spectrometry Analysis of Antioxidant Phenolic Compounds in Salsola komarovii
Authors
이희주판철호김은실김철영
Keywords
antioxidant; flavonoids; phenolic amides; Salsola komarovii
Issue Date
2012-06
Publisher
한국응용생명화학회지; JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
Citation
VOL 55, NO 3, 317-321
Abstract
Online high performance liquid chromatography (HPLC)-2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) based assay system for phenolic antioxidants in Salsola komarovii extracts was applied. HPLC elute was mixed with a stabilized solution of ABTS+ reagents, and the negative peaks, indicating the antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. HPLC with electrospray ionization mass spectrometry was used to identify the seven flavonoids and two phenolic amides in the S. komarovii extracts. The antioxidant compounds were identified as rutin, isoquercitrin, astragalin, and isorhamnetin.
URI
http://pubs.kist.re.kr/handle/201004/42456
ISSN
17382203
Appears in Collections:
KIST Publication > Article
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