Preparative isolation and purification of prenylated isoflavonoids from Cudrania tricuspidata fruits using centrifugal partition chromatography

Title
Preparative isolation and purification of prenylated isoflavonoids from Cudrania tricuspidata fruits using centrifugal partition chromatography
Authors
전제승김상민이희주엄병헌김학기김철영
Keywords
4'-O-methylalpinumisoflavone; 6,8-diprenylgenistein; 6,8-diprenylorobol; centrifugal partition chromatography; Cudrania tricuspidata; prenylated isoflavonoids
Issue Date
2012-06
Publisher
Journal of liquid chromatography & related technologies
Citation
VOL 35, NO 11, 1607-1615
Abstract
Prenylated isoflavonoids such as 6,8-diprenylorobol, 6,8-diprenylgenistein and 4′-O-methylalpinumisoflavone were purified from n-hexane extract of C. tricuspidata fruits using centrifugal partition chromatography (CPC) with a n-hexane-ethyl acetate-methanol-water (7:3:6:4, v/v, ascending mode) solvent system in a one-step within 200 min. One-step preparative CPC yielded 22.4 mg of 6,8-diprenylorobol and 26.7 mg of 6,8-diprenylgenistein with 95% and 85% purity, respectively, from 350 mg of crude hexane extract. Further purification by crystallization in methanol yielded 4′-O-methylalpinumisoflavone (9.3 mg) at over 95% purity based on the HPLC analysis. The chemical structures of purified compounds were elucidated by 1H, 13C NMR, and ESI-MS.
URI
http://pubs.kist.re.kr/handle/201004/42663
ISSN
10826076
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KIST Publication > Article
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