Gravity-oriented microfluidic device for uniform and massive cell spheroid formation

Title
Gravity-oriented microfluidic device for uniform and massive cell spheroid formation
Authors
이강선김충양재영이훈안병욱Linfeng Xu강지윤오광욱
Issue Date
2012-03
Publisher
Biomicrofluidics
Citation
VOL 6, NO 1, 014114-1-014114-7
Abstract
We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours.
URI
http://pubs.kist.re.kr/handle/201004/42699
ISSN
19321058
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KIST Publication > Article
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