Density-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave
- Density-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave
- 남정훈; 임현정; 김충; 강지윤; 신세현
- Issue Date
- VOL 6, NO 2, 024120-1-024120-10
- This study presents a method for density-based separation of monodisperse
encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel.
Even though monodisperse polymer beads can be generated by the state-of-the-art
technology in microfluidics, the quantity of encapsulated cells cannot be controlled
precisely. In the present study, mono-disperse alginate beads in a laminar flow can be
separated based on their density using acoustophoresis. A mixture of beads of equal
sizes but dissimilar densities was hydrodynamically focused at the entrance and then
actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead
is proportional to the density of the bead, i.e., the number of encapsulated cells in an
alginate bead. Under optimized conditions, the recovery rate of a target bead group
(large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per
minute. A cell viability test also confirmed that the encapsulated cells were hardly
damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured
for 1 day were separated in a similar manner. In conclusion, this study demonstrated
that a SSAW can successfully separate monodisperse particles by their density. With
the present technique for separating cell-encapsulating beads, the current cell
engineering technology can be significantly advanced.
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