Characterization and Immunopotentiating Effects of the Glycoprotein Isolated from Dioscorea Batatas

Title
Characterization and Immunopotentiating Effects of the Glycoprotein Isolated from Dioscorea Batatas
Authors
Pham Thi Thu HuongChan-Ho LeeMei Hong LiMin Young Lee김준기Sun-Mee LeeJong-Heum SeonDong-Cheol LeeYoung Jin Jeon
Keywords
Immunopotentiation; Glycoprotein; Macrophages; TNF-α; IL-1β
Issue Date
2011-04
Publisher
The Korean Journal of Physiology & Pharmacology
Citation
VOL 15, NO 2, 101-106
Abstract
We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1β, and TNF-α in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1β, and TNF-α, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.
URI
http://pubs.kist.re.kr/handle/201004/42742
ISSN
12264512
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE