Optimization of pressurized liquid extraction of zeaxanthin from Chlorella ellipsoidea
- Optimization of pressurized liquid extraction of zeaxanthin from Chlorella ellipsoidea
- 구송이; 차광현; 송대근; 정동화; 판철호
- Pressurized liquid extraction; Zeaxanthin; Chlorella ellipsoidea; Optimization
- Issue Date
- Journal of applied phycology
- VOL 24, NO 4, 725-730
- The total zeaxanthin level in Chlorella ellipsoidea,
a green microalga, was more than nine times that of
red pepper, a plant source of zeaxanthin. Additionally, the
zeaxanthin in C. ellipsoidea consisted of the free form,
while those in other plants exist as zeaxanthin mono- and
diesters. Pressurized liquid extraction (PLE) was used to
extract zeaxanthin from C. ellipsoidea. Both the extraction
temperature and extraction time, the two main factors in
PLE, were optimized with a central composite design to
obtain the highest extraction efficiency of zeaxanthin.
Hexane, ethanol, and isopropanol were used as PLE extraction
solvents. Ethanol extracted zeaxanthin most efficiently
from C. ellipsoidea. Temperature was the parameter
with the strongest influence on the extraction of zeaxanthin.
The optimum extraction temperature and time for zeaxanthin
were 115.4°C and 23.3 min, respectively. The
maximum predicted value of 4.28 mg g−1 agreed with the
experimental value of 4.26 mg g−1, supporting the quality
of the fitted model. These results indicate that PLE using
ethanol may be a useful method for extracting zeaxanthin
from C. ellipsoidea.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.