Characteristics of CHO-K1 cells proliferation upon microfluidic media flows

Title
Characteristics of CHO-K1 cells proliferation upon microfluidic media flows
Authors
맹준호정효은신현준정석이상엽
Issue Date
2012-03
Publisher
대한기계학회 바이오공학부문 2012년도 춘계학술대회
Abstract
Microfluidic cell culture chip consisted of two PDMS layer was successfully fabricated using soft lithography. Well structure was applied to isolate shear stress on the bottom of well, and well height was determined by 3D computational model. Fixed number of cells were seeded by cell trap pillar, thus experimental error by cell seeding density could be excluded. In the microfluidic perfusion cell culture, extracellular environment modified by media flow rate affected cell proliferation, viability, and morphological change. When the NDV was 500, the proliferation rate of CHO-K1 cells was significantly enhanced during cultivation of 36 h.
URI
http://pubs.kist.re.kr/handle/201004/42867
Appears in Collections:
KIST Publication > Conference Paper
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