The comparison of microbial community structure in mesophilic biogas reactor by pyrosequencing technology

Title
The comparison of microbial community structure in mesophilic biogas reactor by pyrosequencing technology
Authors
이영행최성아
Keywords
Biogas production; Organic waste; microbial community; Pyrosequencing
Issue Date
2012-06
Publisher
2012 International Conference on Environment and Waste Management
Citation
VOL 66, 1811
Abstract
Variation in taxonomic composition within the piggery was uncovered due to complexity of microbial community, inadequacy of culture dependent microbiological methods. It is important to know about composition and distribution of the microbial community in the piggery and biogas reactor. However, limited information on the archaeal and bacterial composition in the piggery and biogas reactor and to compare the microbial structure is available. We examined 61,424 sequences of microbial 16S rDNA genes by using the pyrosequencing technology to compare the microbial community structures of piggery and biogas reactor (archaea and bacteria). In the bacteria, a core community consisting of 257 OTUs, or 9.54% of the total OTUs, was shared by two samples. The most abundant core Bacteria belonged to three phyla, Unclassified-Bacteria, Bacteroidetes, Firmicutes. Many of sequences 32.42% could be allocated to the phylum Bacteroidetes and the phylum Firmicutes 20.02% is profoundly enriched in the core bacteria community. Otherwise, in the archaea, the overlap for piggery and biogas reactor included 361 shared OTUs. The majority 63.43% of the 361 core OTUs were Methanomicrobiales, which was known to hydrogenotrophic methanogen such as Methanobacteriales. And Methanosarcinales comprised 12.97% that was related to acetoclastic methanogen of shared archaea community. In the archaea, most of sequences are assigned to the Methanomicrobia phylum in the biogas reactor than in the piggery, in particular Methanosarcinales that was related to acetoclastic methanogen. Otherwise, Methanomicrobiales which was known to hydrogenotrophic methanogen are more dominant in the piggery. Most of archaea were allocated to the hyrogenotophic methanogen in the piggery, while acetoclastic methanogen was increased from piggery to biogas reactor. We successfully analyzed both piggery and biogas reactor using taxonomic assignment. Clearly,
URI
http://pubs.kist.re.kr/handle/201004/42954
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KIST Publication > Conference Paper
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