Optimization of simultaneous analysis method for bisphenol A and phthalate metabolites in human breast milk and milk products
- Optimization of simultaneous analysis method for bisphenol A and phthalate metabolites in human breast milk and milk products
- 정민지; 오민석; 이선화; Myeong Hee Moon; Dong Soo Lee; Hye-Jung Yoon; 박경수; 박현미
- simultaneous analysis; milk; bisphenol A; phthalate metabolites
- Issue Date
- Bisphenol A (BPA) and Phthalates are endocrine disrupting chemicals (EDCs) that have been used as compounds in plastics. Daily exposure of humans to BPA and phthalates may be a health risk because these components can affect the function of the reproductive system. Especially, human breast milk is directly exposure source to infants. For these reasons, a method that optimized for instruments and sample preparation was developed for the simultaneous determination of BPA and four phthalate metabolites (mono iso butyl phthalate (MiBP), mono-n-butyl phthalate (MnBP), mono-(2-ethyl-5-oxohexyl)phthalate (MEOHP) and mono-(2-ethyl-5-hydrohexyl)phthalate (MEHHP)) in human breast milk and milk products by GC/TOFMS.
Optimized analytical procedure was based on the incubation for 90minutes at 37℃ with β-glucuronidase/arylsulfatase for enzymatic hydrolysis and used liquid-liquid-extraction with methyl tert butyl ether (MTBE) in pH 7 buffer solution and reacted incubating for 30minutes at 65℃ of derivatization by BSTFA/TMCS(99:1). Analytes were separated using a DB-5MS column (30mx 0.250mm, 0.25μm) and the oven temperature program was as follows; initial 120℃ increased to 200℃ at 20℃/min and hold for 4min, increased to 280℃ at 15℃/min and hold for 2min, increased to 300℃ at 30℃/min and hold for 2min. And the quantitative ions (m/z) for MS were m/z 365, 380 for BPA , m/z 223, 163 for MiBP, m/z 223, 163 for MnBP , m/z 221, 239 for MEOHP and m/z 221, 295 for MEHHP. This optimized method offers to application of real sample via quantitative analysis.
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