Fucoxanthin as a Major Carotenoid in Isochrysis aff. galbana: Characterization of Extraction for Commercial Application

Title
Fucoxanthin as a Major Carotenoid in Isochrysis aff. galbana: Characterization of Extraction for Commercial Application
Authors
김상민강석우권오남정동화판철호
Keywords
carotenoid; extraction; fucoxanthin; Isochrysis aff. galbana; lipid; microalgae; two-phase solvent system
Issue Date
2012-08
Publisher
한국응용생명화학회지; JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
Citation
VOL 55, 477-483
Abstract
Fucoxanthin, a main marine carotenoid, in five species of fucoxanthin-containing microalgae, was quantified by highperformance liquid chromatography. Among the studied species, Isochrysis aff. galbana contained the highest amount of fucoxanthin (18.23 mg/g dried sample). This microalga showed good fucoxanthin extraction efficiency under the tested solvents (methanol, ethanol, acetone, and ethyl acetate), with the exception of n-hexane. In addition, most fucoxanthin (~95%) could be extracted by a single extraction in ethanol within 5 min, and only 15% degradation of fucoxanthin was detected during ethanol extraction for 24 h. The two-phase solvent system of n-hexaneethanol- water with a volume ratio of 10:9:1 was determined to be the best system for the separation of fucoxanthin and lipids from extracts of I. aff. galbana. Under these conditions, fucoxanthin was fractionated in the hydroalcohol phase apart from the hexane phase containing lipids. These results imply that I. aff. galbana can be a commercial source for the spontaneous production of valuable fucoxanthins and lipids.
URI
http://pubs.kist.re.kr/handle/201004/43019
ISSN
17382203
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KIST Publication > Article
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