Isolation of a novel lignocellulose-degrading bacterium
- Isolation of a novel lignocellulose-degrading bacterium
- 공경택; 김세일; 우한민; 엄영순; 박태현
- lignocellulose-degrading bacterium; lignin degradation; kraft lignin
- Issue Date
- International Biotechnology Symposium (IBS) and Exhibition
- Lignocellulosic biomass is a plentiful and useful renewable natural resource that can be used for the production of fuels and chemicals. Microbial degradation of lignocellulosic biomass is one of the promising strategies for producing fuels and chemicals. Lignocellulosic biomass is mainly composed of cellulose, hemicellulose and lignin. General constituents of cellulose, hemicellulose, and lignin are glucose, xylose and aromatic heteropolymer (phenyl propanoid), respectively. The lignin which is the most abundant natural aromatic polymer is well known to be recalcitrant for biodegradation. The microbial degradation of lignin has been studied in white-rot and brown-rot fungi for the last few decades. However, there is very limited information on the degradation of lignin by bacteria compared with fungi. In this study, we report a bacterial strain isolated from Cheonggyesan mountain, Seoul, Korea, which degraded cellulose (carboxymethyl cellulose : CMC), xylan and lignin (kraft lignin). The strain, designated CGM123 is an aerobic and mesophilic. The CGM123 showed the distinct clear zone around the microbial colonies on CMC and xylan agar plates using Gram’s Iodine test. In addition, the CGM123 utilized kraft lignin as a sole carbon source. The CGM123 grew at 0.5 ~ 5 g/l kraft lignin liquid culture medium. The 16S rRNA gene sequence similarity was determined by the EzTaxon database. The phylogenetic analysis showed that strain CGM123 is classified as a novel genus of phylum Bacteroidetes. Strain CGM123 showed highest similarities with Filimonas lacunae (93.30%).
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