Improved intravital microscopy via synchronization of respiration and holder stabilization

Title
Improved intravital microscopy via synchronization of respiration and holder stabilization
Authors
이성온Claudio VinegoniPaolo Fumene FeruglioRalph Weissleder
Keywords
optical microscopy; confocal microscopy; motion compensation; optical imaging
Issue Date
2012-09
Publisher
Journal of biomedical optics
Citation
VOL 17, NO 9, 096018-1-096018-8
Abstract
A major challenge in high-resolution intravital confocal and multiphoton microscopy is physiologic tissue movement during image acquisition. Of the various physiological sources of movement, respiration has arguably the largest and most wide-ranging effect. We describe a technique for achieving stabilized microscopy imaging using a dual strategy. First, we designed a mechanical stabilizer for constraining physical motion; this served to simultaneously increase the in-focus range over which data can be acquired as well as increase the reproducibility of imaging a certain position within each confocal imaging plane. Second, by implementing a retrospective breathing- gated imaging modality, we performed selective image extraction gated to a particular phase of the respiratory cycle. Thanks to the high reproducibility in position, all gated images presented a high degree of correlation over time. The images obtained using this technique not only showed significant improvements over images acquired without the stabilizer, but also demonstrated accurate in vivo imaging during longitudinal studies. The described methodology is easy to implement with any commercial imaging system, as are used by most biological imaging laboratories, and can be used for both confocal and multiphoton laser scanning microscopy.
URI
http://pubs.kist.re.kr/handle/201004/43129
ISSN
10833668
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE