Monitoring bacterial community structure and variability in time scale in full-scale anaerobic digesters
- Monitoring bacterial community structure and variability in time scale in full-scale anaerobic digesters
- 이상훈; 강현진; 이영행; 이택준; 한금석; 최영준; 박희등
- Biogas production; Anaerobic digesters; Bacterial Community; Pyrosequencing
- Issue Date
- Journal of environmental monitoring : JEM
- VOL 14, NO 7, 1893-1905
- Using a high-throughput pyrosequencing technology, this study assessed bacterial community
structure and time-scale variability in great detail in seven full-scale anaerobic digesters operated
variously in terms of influent substrate, digestion temperature, and reactor configuration.
Pyrosequencing generated a total of 83 774 sequence reads from 40 digester sludge samples collected
monthly for six months. The highest number of sequence reads were detected within Proteobacteria
(20.5%), followed by those within Bacteroidetes (19.7%), Firmicutes (17.8%), and Chloroflexi (4.8%).
The relative composition of bacterial populations was varied within the digesters as well as between the
digesters, and the bacterial community structures were mainly influenced by digestion temperature.
Detailed bacterial community structures were assessed by analyzing the operational taxonomic units
(OTUs) based on 97% sequence similarity, which resulted in a total of 9051 OTUs. Among these, a total
of 31 core OTUs were analyzed and inferred phylogenetically, which enabled us to classify the
sequences within an unclassified phylum. Unclassified sequences were mostly affiliated with the
sequences within Spirochaetes and Firmicutes. Interestingly, numerically dominant novel phylotypes
(18% of the total sequence reads) presumably involved in anaerobic digestion within Spirochaetes were
identified. Temporal variability was further explored using a non-metric multidimensional scaling
ordination which demonstrated that the variability of the bacterial community within the digesters was
smaller than between digesters. Correlation analysis demonstrated that digester performance and
operational conditions affected the pattern of bacterial community in the ordination. Additionally,
a multi-response permutation procedure revealed that the bacterial communities within the digesters
were more similar than those belonging to other digeste
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