Ultrafiltration LC-MS for high throughput screening of natural products to various molecular targets

Title
Ultrafiltration LC-MS for high throughput screening of natural products to various molecular targets
Authors
최용수Richard B. van Breemen
Keywords
Ultrafiltration; LC-MS; High throughput screening; natural products
Issue Date
2012-08
Publisher
한국질량분석학회 2012여름정기학술대회
Citation
, 33-33
Abstract
High throughput screening (HTS) is a crucial technology in drug discovery, which has been routinely used since 1990s as pharmaceuticals hold dramatically increasing number of their own libraries (> several million compounds). Conventional HTS based on spectrometric detection, however, has a serious drawback when applied for drug discovery in natural products. Unlike synthetic single compound, natural product extracts contains complex matrix and multiple compounds which show too many false positives. Aff,rnity based mass spectrometric technique can be avery altraclive screening method because of its selectivity and sensitivity. In this presentation, a high throughput ultrafiltration LC-MS and MALDI- MS techniques will be introduoed to screen natural products for bioactive compounds to several macromolecular targets, such as quinon reductase-2, Keap-1, estrogen receptors, retinoid X receptors, and vitamin D receptor. Screening results obtained by these techniques demonstrated that affinity based MS can be used for screening complex natural products targeted to many other enzymes and proteins.
URI
http://pubs.kist.re.kr/handle/201004/43172
Appears in Collections:
KIST Publication > Conference Paper
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