Pinusolide Isolated from Biota orientalis Inhibits 5-Lipoxygenase Dependent Leukotriene C4 Generation by Blocking c-Jun N-Terminal Kinase Pathway in Mast Cells

Title
Pinusolide Isolated from Biota orientalis Inhibits 5-Lipoxygenase Dependent Leukotriene C4 Generation by Blocking c-Jun N-Terminal Kinase Pathway in Mast Cells
Authors
Ye Jin양현옥손종근장현욱
Keywords
Biota orientalis; Pinusolide; bone marrow-derived mast cell; leukotriene C4; cytosolic phospholipase A2; c-Jun N-terminal kinase
Issue Date
2012-08
Publisher
Biological & pharmaceutical bulletin
Citation
VOL 35, NO 8, 1374-1378
Abstract
Pinusolide, an herbal medicine isolated from Biota orientalis L. (B. orientalis), inhibited 5-lipoxygenase (5-LO)-dependent leukotriene C4 (LTC4) generation in immunoglobulin E (IgE)/Ag-induced bone marrowderived mast cells (BMMCs) in a concentration-dependent manner. To clarify the action mechanism of pinusolide on the inhibition of LTC4 generation, we examined the effect of pinusolide on phosphorylation of cytosolic phospholipase A2 (cPLA2), as well as translocation phospho-cPLA2 and 5-LO to nucleus. Inhibition of LTC4 generation by pinusolide was accompanied by a decrease in cPLA2 phosphorylation which occurred via a decrease in intracellular Ca2 influx and blocking the c-Jun N-terminal kinase (JNK) pathways. However, pinusolide had no effect on extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinas phosphorylation. Taken together, the present results suggest that potent inhibition of 5-LO dependent LTC4 generation by pinusolide requires both suppression of calcium influx and JNK phosphorylation.
URI
http://pubs.kist.re.kr/handle/201004/43184
ISSN
09186158
Appears in Collections:
KIST Publication > Article
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