Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis

Title
Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis
Authors
조현종윤홍열구희범고성학심재성조희현박재형김광명권익찬김대덕
Keywords
Hyaluronic acid-ceramide; MRI; NIRF; Dual modality; Tumor targeting
Issue Date
2012-08
Publisher
Journal of controlled release
Citation
VOL 162, NO 1, 111-118
Abstract
Hyaluronic acid-ceramide (HACE)-based nanoprobes for magnetic resonance (MR) and optical imaging were developed for cancer diagnosis. Diethylenetriaminepentaacetic dianhydride (DTPA) was conjugated to HACE for the chelation of gadolinium (Gd) as an MR contrast agent. Cy5.5 was also conjugated to the HACE backbone as a near-infrared fluorescence (NIRF) imaging dye. The self-assembled HACE-based nanoprobe, Cy5.5-HACE-DTPA-Gd, exhibited a uniformly distributed particle size and morphological shape. The HACEbased nanoprobe did not induce serious cytotoxicity in U87-MG (low expression of CD44 receptor) and SCC7 (high expression of CD44 receptor) cells. The cellular uptake efficiency of the HACE-based nanoprobe was higher in SCC7 cells than in U87-MG cells, indicating an HA-CD44 receptor interaction. In vitro MR signal enhancement of the HACE-based nanoprobe was confirmed compared with a commercial formulation (Magnevist). Moreover, in vivo MR contrast enhancement of the HACE-based nanoprobe in the tumor region was verified in an SCC7 tumor xenograft mouse model. The tumor targetability of the developed nanoprobe was monitored by an NIRF imaging study, and improved accumulation of the nanoprobe in the tumor region was observed. Therefore, this HACE-based dual-imaging nanoprobe can be used to make a more accurate diagnosis of cancer based on its passive and active tumor targeting strategies.
URI
http://pubs.kist.re.kr/handle/201004/43315
ISSN
01683659
Appears in Collections:
KIST Publication > Article
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