Isolation and Characterization of a Novel Bacterial Strain for Lignin Degradation

Title
Isolation and Characterization of a Novel Bacterial Strain for Lignin Degradation
Authors
공경택김세일우한민박태현엄영순
Keywords
lignin degradation; lignin degrading bacteria; kraft lignin
Issue Date
2012-10
Publisher
Lignobiotech II
Abstract
In this study, we report an aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated as CGM1-3ENT, isolated from the enrichment cultures of forest soil from Cheonggysan Mountain, Republic of Korea. The cells were gram-negative and motile rod (1.3-2.4 3m long by 0.30-0.75 3m wide) with single flagella. The strain grows at temperatures between 10 and 37 °C (optimum: 25-37 °C) and at pH levels of 4.5-9.5 (optimum: 5.0-7.0). The major fatty acids were C16:0, C18:1, and C18:1 w7c 11-methyl. The strain CGM1-3ENT used D-glucose, D-fructose, sucrose, maltose, trehalose, D-mannose, D-mannitol, D-sorbitol, D-galactose, D-cellobiose, lactose, D-raffinose, fumarate, pyruvate, and DL-alanine as carbon sources. Interestingly, the strain was able to grow in a medium containing 0.5 ~ 5 g/L kraft lignin as a sole carbon source. The genomic DNA G+C content of the type strain is 63.7 mol%. The closest phylogenetic neighbor to the strain CGM1-3ENT was identified as Asticcacaulis biprosthecium DSM 4723T (97.1% 16S rRNA gene similarity). Based on the polyphasic evidence presented in this study, the forest soil isolate CGM1-3ENT was proposed to be classified as a novel species in the genus Asticcacaulis and to be named Asticcacaulis silvisoli sp. nov.
URI
http://pubs.kist.re.kr/handle/201004/43369
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KIST Publication > Conference Paper
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