Gold Nanoparticles for Cancer Imaging and Therapy
- Gold Nanoparticles for Cancer Imaging and Therapy
- 선인철; 구희범; 류주희; 이동기; 권익찬; 안철희; 김광명
- gold nanoparticle; CT; optical imaging; dual imaging probe
- Issue Date
- Purpose : We developed a matrix metalloproteinase (MMP)-sensitive gold nanorods (AuNR) for cancer imaging/therapy and tumor-targeting dual (optical/CT) imaging probe with gold nanoparticles (AuNPs).
Method : Glycol chitosan (GC) was used as a reducing agent and stabilizer of AuNPs to improve the stability and tumor-targeting property. Cy5.5 fluorescent dye and black hole quencher were conjugated to MMP substrate peptide and the other end of peptide was chemically linked to GC coated AuNPs. In addition, these peptides were conjugated to the AuNR for imaging and therapy.
Results :These particles (BCMG-AuNP) showed more excellent stability in the physiological condition than uncoated AuNPs. The X-ray absorption property of gold remained after the surface coating with GC and peptide conjugation, which enabled the anatomical in vivo imaging of tumor by CT due to the selective accumulation of probes in tumor tissue. The near infrared fluorescence (NIRF) of BCMG-AuNPs was effectively quenched by the combinational quenching system and the quenched fluorescence was recovered by MMP enzymes, which makes it possible to detect nanomolar amounts of protease, both in vitro and in vivo. When the peptides were conjugated to AuNRs, they successfully imaged cancer with fluorescence and therapeutic effect was seen by laser irradiation
Conclusion :Therefore, this BCMG-AuNP probe was expected to provide the platform of multimodal imaging probe for cancer detection and various applications such as drug screening or monitoring for therapeutic efficacy in vivo. Also when we used AuNR, we were able to combine imaging and therapy at the same time.
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