Tumor-targeting gold particles for CT/optical dual cancer imaging

Tumor-targeting gold particles for CT/optical dual cancer imaging
gold nanoparticle; CT; optical imaging; dual imaging probe
Issue Date
2012 The World Molecular Imaging Congress (WMIC)
We developed a matrix metalloproteinase (MMP)-sensitive, tumor-targeting imaging probe with gold nanoparticles (AuNPs) for fluorescence optical imaging and computed tomography (CT). Glycol chitosan (GC) was used as a reducing agent and stabilizer of AuNPs to improve the stability and tumortargeting properties. Cy5.5 fluorescent dye and black hole quencher were conjugated to MMP substrate peptide and the other end of peptide was chemically linked to GC coated AuNPs (BCMG-AuNP). Those particles showed excellent stability in the physiological condition and this enabled BCMG-AuNP to absorb X-ray more intensively. The tumor tissues were imaged with the probe through CT and optical imaging. We were able to obtain the anatomical in vivo imaging of tumor through CT due to the selective accumulation of probes in tumor tissues. In addition, The near infrared fluorescence (NIRF) of BCMG-AuNPs was effectively quenched by the combinational quenching system and the quenched fluorescence was recovered by MMP enzymes, which makes it possible to detect tumors as well as nanomolar amounts of protease, both in vitro and in vivo. Therefore, this BCMG-AuNP probe was expected to provide the platform of multimodal imaging probe for cancer detection and various applications such as drug screening or monitoring for therapeutic efficacy in vivo.
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