Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry
- Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry
- 강운범; 염정훈; 김혜정; 김호근; 이철주
- Quantitative analysis; mTRAQ; cICAT; Colorectal cancer; Stable isotope labeling; Mass spectrometry
- Issue Date
- JOURNAL OF PROTEOMICS
- VOL 75, NO 10, 3050-3062
- An efficient means of identifying protein biomarkers is essential to proper cancer
management. A well-characterized proteome resource holds special promise for the discovery
of novel biomarkers. However, quantification of the differences between physiological conditions
together with deep down profiling has become increasingly challenging in proteomics.
Here,we performexpression profiling of the colorectal cancer (CRC) proteome by stable isotope
labeling andmass spectrometry. Quantitative analysis included performingmTRAQ and cICAT
labeling in a pooled sample of three microsatellite stable (MSS) type CRC tissues and a pooled
sample of theirmatched normal tissues.We identified and quantified a total of 3688 proteins.
Among them, 1487 proteins were expressed differentially between normal and cancer tissues
by higher than 2-fold; 1009 proteins showed increased expression in cancer tissue, whereas
478 proteins showed decreased expression. Bioinformatic analysis revealed that our data
were largely consistentwith known CRC relevant signaling pathways, such as theWnt/β-catenin,
caveolar-mediated endocytosis, and RAN signaling pathways. Mitochondrial dysfunction,
known as theWaburg hypothesis,was also confirmed. Therefore, our data showing alterations
in the proteomic profile of CRC constitutes a useful resource that may provide insights into
tumor progression with later goal of identifying biologically and clinically relevantmarker proteins.
This article is part of a Special Issue entitled: Proteomics: The clinical link.
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