Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry

Title
Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry
Authors
강운범염정훈김혜정김호근이철주
Keywords
Quantitative analysis; mTRAQ; cICAT; Colorectal cancer; Stable isotope labeling; Mass spectrometry
Issue Date
2012-06
Publisher
JOURNAL OF PROTEOMICS
Citation
VOL 75, NO 10, 3050-3062
Abstract
An efficient means of identifying protein biomarkers is essential to proper cancer management. A well-characterized proteome resource holds special promise for the discovery of novel biomarkers. However, quantification of the differences between physiological conditions together with deep down profiling has become increasingly challenging in proteomics. Here,we performexpression profiling of the colorectal cancer (CRC) proteome by stable isotope labeling andmass spectrometry. Quantitative analysis included performingmTRAQ and cICAT labeling in a pooled sample of three microsatellite stable (MSS) type CRC tissues and a pooled sample of theirmatched normal tissues.We identified and quantified a total of 3688 proteins. Among them, 1487 proteins were expressed differentially between normal and cancer tissues by higher than 2-fold; 1009 proteins showed increased expression in cancer tissue, whereas 478 proteins showed decreased expression. Bioinformatic analysis revealed that our data were largely consistentwith known CRC relevant signaling pathways, such as theWnt/β-catenin, caveolar-mediated endocytosis, and RAN signaling pathways. Mitochondrial dysfunction, known as theWaburg hypothesis,was also confirmed. Therefore, our data showing alterations in the proteomic profile of CRC constitutes a useful resource that may provide insights into tumor progression with later goal of identifying biologically and clinically relevantmarker proteins. This article is part of a Special Issue entitled: Proteomics: The clinical link.
URI
http://pubs.kist.re.kr/handle/201004/43783
ISSN
18743919
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KIST Publication > Article
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