Comparative phosphoproteome analysis reveals more ERK activation in MDA-MB-231 than in MCF-7
- Comparative phosphoproteome analysis reveals more ERK activation in MDA-MB-231 than in MCF-7
- Mohammad Humayun Kabir; 서의진; 이철주
- Phosphoprotein; TiO2; MCF-7; MDA-MB-231; ERK; Phosphorylation motif
- Issue Date
- International journal of mass spectrometry
- VOL 309, 1-12
- Protein phosphorylation forms the foundation of many intra-cellular signaling networks. Enrichment is
very important to analyze phosphoproteins due to their low abundance in cells. We used TiO2 enrichment
and LC–MS/MS to identify phosphopeptides from two breast cancer cells, MCF-7 and MDA-MB-231. The
dataset generated were searched using Sequest algorithm with 1% false discovery rate. It was observed
that 234 phosphoproteins and 280 phosphopeptides were common from a total of 590 and 455 phosphoproteins
and 802 and 599 non-redundant phosphopeptides identified from MCF-7 and MDA-MB-231,
respectively. Besides, a total of 843 and 578 non-redundant phosphorylation sites were also identified
from these two cell lines. ERK1/ERK2 kinase substrate motif (PX[S/T]#P) was identified in MDA-MB-231
but not in MCF-7, showing that this kinase signal transduction pathway is a key factor during breast
cancer progression. Activation of ERK1/ERK2 in MDA-MB-231 was further confirmed by Western blot.
Quantitative analysis by spectral counting revealed 31 phosphoproteins were statistically in different
levels between the two cells lines. This difference is due to the differences in kinase activities as well as
protein expression levels. Since breast cancer research makes extensive use of both of these cell lines,
our study’s findings will be of value in understanding phosphorylation signal of breast cancer.
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