Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer

Title
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer
Authors
정지한김혜정염정훈유창영신지혜유진영강창숙이철주
Keywords
Galectin-2; Gastric cancer; ICAT; Mass spectrometry; Immunohistochemistry
Issue Date
2012-01
Publisher
Journal of gastroenterology
Citation
VOL 47, NO 1, 37-48
Abstract
Background Lymph node metastasis (LNM) is recognized as an important factor in the progression of tumor malignancy. It is required to discover molecular markers for the prediction of LNM in gastric cancers (GCs). Methods An isotope coded affinity tag (ICAT) method and mass spectrometry were used for the quantitative profiling of LNM-related proteins. Western blot analysis of the identified proteins and immunohistochemistry on a tissue microarray comprising 120 GC cases were performed for validation. Results We identified 151 differentially expressed proteins (DEPs) with an abundance ratio greater than 1.5-fold. The proteins upregulated in LNM-negative GCs were largely populated with proteins related to cell death. Among the DEPs, galectin-2 was further tested because its expression level was significantly higher in LNM-negative GCs (*12-fold, p\0.0001) and its expression is known to be not ubiquitous but confined to the gastrointestinal tract. Immunohistochemical analysis revealed that low expression of galectin-2 was significantly associated with LNM (p = 0.031) and advanced clinical stage (p = 0.024). The association of low galectin-2 with LNM was found even in early GCs (p = 0.020). Conclusion Our results show that proteomic analysis coupled with immunohistochemistry using tissue microarray is a useful tool for identifying LNM-associated proteins in GC. Also, loss of galectin-2 might play an important role in the aggressiveness of GC.
URI
http://pubs.kist.re.kr/handle/201004/43786
ISSN
09441174
Appears in Collections:
KIST Publication > Article
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