Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer
- Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer
- 정지한; 김혜정; 염정훈; 유창영; 신지혜; 유진영; 강창숙; 이철주
- Galectin-2; Gastric cancer; ICAT; Mass spectrometry; Immunohistochemistry
- Issue Date
- Journal of gastroenterology
- VOL 47, NO 1, 37-48
- Background Lymph node metastasis (LNM) is recognized
as an important factor in the progression of tumor
malignancy. It is required to discover molecular markers
for the prediction of LNM in gastric cancers (GCs).
Methods An isotope coded affinity tag (ICAT) method
and mass spectrometry were used for the quantitative
profiling of LNM-related proteins. Western blot analysis of
the identified proteins and immunohistochemistry on a
tissue microarray comprising 120 GC cases were performed
Results We identified 151 differentially expressed proteins
(DEPs) with an abundance ratio greater than 1.5-fold.
The proteins upregulated in LNM-negative GCs were largely
populated with proteins related to cell death. Among
the DEPs, galectin-2 was further tested because its
expression level was significantly higher in LNM-negative
GCs (*12-fold, p\0.0001) and its expression is known
to be not ubiquitous but confined to the gastrointestinal
tract. Immunohistochemical analysis revealed that low
expression of galectin-2 was significantly associated with
LNM (p = 0.031) and advanced clinical stage (p = 0.024).
The association of low galectin-2 with LNM was found
even in early GCs (p = 0.020).
Conclusion Our results show that proteomic analysis
coupled with immunohistochemistry using tissue microarray
is a useful tool for identifying LNM-associated proteins
in GC. Also, loss of galectin-2 might play an
important role in the aggressiveness of GC.
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