Early diagnosis and progression visualization of rheumatoid arthritis using fluorogenic MMP-3 polymeric probes
- Early diagnosis and progression visualization of rheumatoid arthritis using fluorogenic MMP-3 polymeric probes
- 류주희; 이애주; 고창용; 김한성; 권익찬; 윤인찬; 김광명
- Issue Date
- 2012 The World Molecular Imaging Congress (WMIC)
- Early start of treatment through an early diagnosis of rheumatoid arthritis (RA) could stop progression of the disease, but the early diagnosis is often associated with difficulties. Matrix metalloproteinase-3 (MMP-3) is thought to be notably important in the progression of RA. The MMP-3 polymeric probe was made by conjugating near infrared fluorescent dye, dark quencher, and MMP-3 substrate peptide, to chitosan nanoparticles. The use of fluorogenic MMP-3 polymeric probe injected intravenously, enabled a clear diagnosis of RA at two weeks after the first immunization and before any signals of anatomical or structural changes could be detected with the naked eye or micro-CT and histological analyses. Furthermore, MMP-3 polymeric probe enabled visualization progression of RA progression using near infrared fluorescent optical imaging system. The results of Western blotting and immunohistochemistry revealed the recovered fluorescence in in vivo imaging was attributed to the up-regulated MMP-3 activity in the arthritic joints. Therefore, this approach could be used for therapeutic monitoring and early diagnosis.
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