ATP-sensitive potassium channels exhibit variance in the number of open channels below the limit predicted for identical and independent gating
- ATP-sensitive potassium channels exhibit variance in the number of open channels below the limit predicted for identical and independent gating
- 최기현; Stuart Licht
- Issue Date
- PLoS ONE
- VOL 7, NO 5, e37399-1-e37399-6
- In small cells containing small numbers of ion channels, noise due to stochastic channel opening and closing can introduce a substantial level of variability into the cell's membrane potential. Negatively cooperative interactions that couple a channel's gating conformational change to the conformation of its neighbor(s) provide a potential mechanism for mitigating this variability, but such interactions have not previously been directly observed. Here we show that heterologously expressed ATP-sensitive potassium channels generate noise (i.e., variance in the number of open channels) below the level possible for identical and independent channels. Kinetic analysis with single-molecule resolution supports the interpretation that interchannel negative cooperativity (specifically, the presence of an open channel making a closed channel less likely to open) contributes to the decrease in noise. Functional coupling between channels may be important in modulating stochastic fluctuations in cellular signaling pathways.
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