Coupling of ryanodine receptor 2 and voltage-dependent anion channel 2 is essential for Ca2+ transfer from the sarcoplasmic reticulum to the mitochondria in the heart

Title
Coupling of ryanodine receptor 2 and voltage-dependent anion channel 2 is essential for Ca2+ transfer from the sarcoplasmic reticulum to the mitochondria in the heart
Authors
민준기염동림이경은권혜경Moonkyung KangYeon-Soo KimZee Yong Park전혜성Do Han Kim
Keywords
cardiomyocyte; high Ca2+ microdomain; interorganelle Ca2+ transfer; mitochondrial Ca2+ uptake; ryanodine receptor (RyR); sarcoplasmic reticulum?mitochondria junction
Issue Date
2012-11
Publisher
The Biochemical journal
Citation
VOL 447, NO 3, 371-379
Abstract
The structural proximity and functional coupling between the SR (sarcoplasmic reticulum) and mitochondria have been suggested to occur in the heart. However, themolecular architecture involved in the SR–mitochondrial coupling remains unclear. In the present study, we performed various genetic and Ca2+ -probing studies to resolve the proteins involved in the coupling process. By using the bacterial 2-hybrid, glutathione transferase pull-down, co-immunoprecipitation and immunocytochemistry assays, we found that RyR2 (ryanodine receptor type 2), which is physically associated with VDAC2 (voltage-dependent anion channel 2), was co-localized in SR–mitochondrial junctions. Furthermore, a fractionation study revealed that VDAC2 was co-localized with RyR2 only in the subsarcolemmal region. VDAC2 knockdown by targeted short hairpin RNA led to an increased diastolic [Ca2+ ] (calcium concentration) and abolishment of mitochondrial Ca2+ uptake. Collectively, the present study suggests that the coupling of VDAC2 with RyR2 is essential for Ca2+ transfer from the SR to mitochondria in the heart.
URI
http://pubs.kist.re.kr/handle/201004/43841
ISSN
02646021
Appears in Collections:
KIST Publication > Article
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