Efficient Separation of Coronary Artery Disease Biomarkers via Colloidal Crystallization of Magnetic Nanoparticles

Title
Efficient Separation of Coronary Artery Disease Biomarkers via Colloidal Crystallization of Magnetic Nanoparticles
Authors
박호영김태호황민태김유찬석현광현택환이관희
Keywords
Colloidal; Magnetic Particle; Coronary Artery Disease
Issue Date
2012-04
Publisher
대한금속재료학회
Abstract
In this study, we hypothesize that superparamagnetic colloidal-crystallized magnetic nanoparticles(CCNPs) with good water dispersity can be used to detect and separate an early-stage biomarker for CAD (C-reactive protein or CRP). To synthesize CCNPs, we used an oil-in-water microemulsion system. Magnetic particles (MBs) and nanoparticles (NPs) were used as controls. The structure and size distribution of the particles(MBs, NPs, CCNPs) were characterized using TEM and DLS. To confirm the potential to separate the particles from solution, we used a magnet and took optical images. Finally, antibodies specific for (CRP) were conjugated onto the particles (MBs, NPs, CCNPs) using SMCC chemistry. The functionality of the particles was tested by incubating them with biomarkers for CRP in PBS, magnetically separating them, and analyzing the subsequent levels of CRP. We conclude that superparamagnetic CCNPs with good water dispersity are an effective alternative to current methods in the separation of CAD biomarkers.
URI
http://pubs.kist.re.kr/handle/201004/43884
Appears in Collections:
KIST Publication > Conference Paper
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