Efficient Separation of Coronary Artery Disease Biomarkers via Colloidal Crystallization of Magnetic Nanoparticles
- Efficient Separation of Coronary Artery Disease Biomarkers via Colloidal Crystallization of Magnetic Nanoparticles
- 박호영; 김태호; 황민태; 김유찬; 석현광; 현택환; 이관희
- Colloidal; Magnetic Particle; Coronary Artery Disease
- Issue Date
- In this study, we hypothesize that superparamagnetic colloidal-crystallized magnetic nanoparticles(CCNPs) with good water dispersity can be used to detect and separate an early-stage biomarker for CAD (C-reactive protein or CRP). To synthesize CCNPs, we used an oil-in-water microemulsion system. Magnetic particles (MBs) and nanoparticles (NPs) were used as controls. The structure and size distribution of the particles(MBs, NPs, CCNPs) were characterized using TEM and DLS. To confirm the potential to separate the particles from solution, we used a magnet and took optical images. Finally, antibodies specific for (CRP) were conjugated onto the particles (MBs, NPs, CCNPs) using SMCC chemistry. The functionality of the particles was tested by incubating them with biomarkers for CRP in PBS, magnetically separating them, and analyzing the subsequent levels of CRP. We conclude that superparamagnetic CCNPs with good water dispersity are an effective alternative to current methods in the separation of CAD biomarkers.
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