Quantitative Molecular Detection of Pancreatic Cancer Target Biomarkers Using Highly Sensitive Hybrid Nanoprobes
- Quantitative Molecular Detection of Pancreatic Cancer Target Biomarkers Using Highly Sensitive Hybrid Nanoprobes
- Pancreatic Cancer; Quantum Dots; Nanoprobes; Ferritin
- Issue Date
- IEEE-NANOMED 2012
- Pancreatic cancer is the fourth leading cause of cancer death in the United States. Perhaps more shocking is that among all cancers, pancreatic cancer has the highest ratio between death rate and incidence rate. The primary reason behind this statistic is that a large fraction (about 80%) of tumors is metastatic at the time of diagnosis. Therefore, the early detection of pancreatic cancer is of utmost importance to improve the survival rate of patients. The sequential appearance of molecular abnormalities during the progression of PanINs (Pancreatic Intraepithelial Neoplasia) to invasive and metastatic pancreatic cancer is a promising avenue for early detection. The absolute amount of such biomarkers, however, is quite often too low to detect using standard methods. We thus propose a signal amplification method to detect and quantitate small signals. We used a bacterial expression system to synthesize multifunctional nanostructures, which consist of 24 self-assembled human ferritin heavy chain (hFTN-Hs) subunits and a linker peptide on each subunit. The activity of the self-assembled hFTN-Hs leads to the formation of spherical nanoparticles surface-labeled with linker peptide. Lipid-coated quantum dots (QDs) that are thiol-modified were chemically conjugated to amine functional groups on the linker protein. Likewise, an anti-pancreatic cancer cell antibody was conjugated onto the nanoparticle. Consequently, we were able to obtain an amplified signal, which could be used in the early detection of pancreatic cancer biomarkers.
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