Ethanol-Dispersed Polymer Nanofi bers as a Highly Selective Cell Isolation and Release Platform for CD4 + T Lymphocytes

Title
Ethanol-Dispersed Polymer Nanofi bers as a Highly Selective Cell Isolation and Release Platform for CD4 + T Lymphocytes
Authors
전승현김광희안효진김병찬손정희김미주도준상Yee, Cassian이경미김중배
Keywords
Nanofiber; Receptor; CD4+T Lymphocytes; Isolation; Release
Issue Date
2012-11
Publisher
Advanced functional materials
Citation
VOL 22, NO 21, 4448-4455
Abstract
A novel cell isolation and release platform using electrospun polystyrenepoly( styrene- co -maleic anhydride) (PS-PSMA) nanofi bers is presented. Ethanol treatment of PS-PSMA nanofi bers, employed for the purpose of sterilization, signifi cantly increases their inter-fi ber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4 + T cells from heterogeneous mixtures of mouse lymph nodes, capture effi ciencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4 + T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the fi rst demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4 + T cells for the purpose of cell therapies.
URI
http://pubs.kist.re.kr/handle/201004/43947
ISSN
1616301X
Appears in Collections:
KIST Publication > Article
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