A compound isolated from the rhizomes of Reynoutria elliptica protects transformed retinal ganglion cells (RGC-5) against oxidative stress in vitro and in vivo

Title
A compound isolated from the rhizomes of Reynoutria elliptica protects transformed retinal ganglion cells (RGC-5) against oxidative stress in vitro and in vivo
Authors
조형이희주김철영김경아손진기정상훈
Keywords
neuroprotection; retinal ganglion cell; oxidative stress; glaucoma; Reynoutria elliptica; 8-hydroxycalamenene; RGC-5
Issue Date
2012-09
Publisher
International Biotechnology Symposium (IBS) and Exhibition
Abstract
This study was to determine whether a compound isolated from Reynoutria elliptica could attenuate oxidative stress-induced RGC-5 death. Among the isolated compounds from R. elliptica, 8-hydroxycalamenene attenuated the cell death of transformed RGC-5 cells. This compound also produced a dose-dependent decrease in the expression of apoptotic proteins (cleaved PARP and caspase-3) induced by glutamate/BSO and stimulated glutathione and glutathione S-transferase activity. Moreover, the addition of 8-hydroxycalamenene to cell cultures restored the reduced mitochondrial membrane potential caused by glutamate/BSO treatment. The presence of N-methyl-D-aspartate (NMDA) in the retina of rats affected the thickness of the inner plexiform layer (IPL) and increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) positive RGCs. However, 8-hydroxycalamenene protected against thinning of the IPL and reduced TUNEL-positive cells in the ganglion cell layer. In conclusion, 8-hydroxycalamenene isolated from R. elliptica exerts neuroprotective effects both in vitro and in vivo.
URI
http://pubs.kist.re.kr/handle/201004/43970
Appears in Collections:
KIST Publication > Conference Paper
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