A compound isolated from the rhizomes of Reynoutria elliptica protects transformed retinal ganglion cells (RGC-5) against oxidative stress in vitro and in vivo
- A compound isolated from the rhizomes of Reynoutria elliptica protects transformed retinal ganglion cells (RGC-5) against oxidative stress in vitro and in vivo
- 조형; 이희주; 김철영; 김경아; 손진기; 정상훈
- neuroprotection; retinal ganglion cell; oxidative stress; glaucoma; Reynoutria elliptica; 8-hydroxycalamenene; RGC-5
- Issue Date
- International Biotechnology Symposium (IBS) and Exhibition
- This study was to determine whether a compound isolated from Reynoutria elliptica could attenuate oxidative stress-induced RGC-5 death.
Among the isolated compounds from R. elliptica, 8-hydroxycalamenene attenuated the cell death of transformed RGC-5 cells.
This compound also produced a dose-dependent decrease in the expression of apoptotic proteins (cleaved PARP and caspase-3) induced by glutamate/BSO and stimulated glutathione and glutathione S-transferase activity. Moreover, the addition of 8-hydroxycalamenene to cell cultures restored the reduced mitochondrial membrane potential caused by glutamate/BSO treatment.
The presence of N-methyl-D-aspartate (NMDA) in the retina of rats affected the thickness of the inner plexiform layer (IPL) and increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) positive RGCs. However, 8-hydroxycalamenene protected against thinning of the IPL and reduced TUNEL-positive cells in the ganglion cell layer.
In conclusion, 8-hydroxycalamenene isolated from R. elliptica exerts neuroprotective effects both in vitro and in vivo.
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