Relative metabolic rates of di(2-ethylhexyl) phthalate between South Koreans in relation to age

Relative metabolic rates of di(2-ethylhexyl) phthalate between South Koreans in relation to age
relative metabolic rate; urinary DEHP metabolites; DEHP metabolism
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Di(2-ethylhexyl) phthalate (DEHP) is one of commonly used plasticizers along with diisodecyl phthalate and diisononyl phthalate in manufacturing plastics, mainly polyvinyl chloride, and makes up the products from 1 % up to 40 %. The present study was aimed to monitor the relative metabolic rates (RMR) of DEHP between South Koreans through its urinary metabolites: mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5-OH-MEHP) and mono(2-ethyl-5-oxohexyl) phthalate (5-oxo-MEHP) in human references (n = 1642) that were collected by Korea Food and Drug Administration between May 2011 and September 2011 survey years. Liquid-liquid extraction was performed in order to simultaneously analyze all three analytes. Trimethylsilylation derivatization was employed and its derivatives were analyzed using isotope dilution gas chromatography-mass spectrometry method. Qualitative analysis of each metabolite was carried out with corresponding isotope labeled internal standards and method detection limit ranged from 0.05 ng/mL to 0.2 ng/mL. Calibration curves were drawn between 0.2 ng/mL and 100.0 ng/mL and resulting linearity ranged from 0.9992 to 0.9999. Samples that have 100.0 % detection frequency for all three DEHP metabolites were chosen and categorized in to adults, students and children. To minimize the statistical influence from extreme values, 95.0 % (2.5 % - 97.5 %) confidence interval of each group was further selected and fractionation of DEHP metabolites and RMR of each step of DEHP metabolism were calculated. MEHP fractionation percentage increased in order of children, student and adults, respectively. In other words, the RMR of the first step of DEHP metabolism (RMR1 depicts hydroxylation of MEHP to 5-OH-MEHP) increased in order of adults, students and children, respectively.
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