Determination of oxysterols in human serum by liquid chromatography-silver coordination ion spray/tandem mass spectrometry(LC-AG+CIS/MS/MS)

Title
Determination of oxysterols in human serum by liquid chromatography-silver coordination ion spray/tandem mass spectrometry(LC-AG+CIS/MS/MS)
Authors
차은주김소희김희원김호준손정현권오승이재익
Issue Date
2012-11
Publisher
한국분석과학회
Abstract
Oxysterols are mono-oxygenated forms of cholesterol and play an important role for preserving lipid homeostasis in the body. Recently, several study for cholesterols in biological samples were reported using liquid chromatography-tandem mass spectrometry equipped electrospray ionization or atmospheric pressure chemical ionization instead of the gas chromatography-mass spectrometry. However the lacks of ionization sites of cholesterols have restricted the sensitivity and reproducibility of analytical method. Therefore, we have developed a highly sensitive and specific method using Ag+ coordination ion spray ionization (CIS) to analyze the oxysterols such as 24S-hydroxycholesterol (24S-OHC), 25-hydroxycholesterol (25-OHC) or 27-hydroxycholesterol (27-OHC) in human serum. Calibration curves were obtained over a linear range of 5 to 250 ng/mL for 24S-OHC and 27-OHC, and 0.5 to 25 ng/mL for 25-OHC. Intra- and inter-day precisions were between 1.0% and 13.3% and the accuracies ranged from 94.3% to 109.0%. Limit of detection and limit of quantification were 0.3 ng/mL, 5 ng/mL for 24S-OHC and 27-OHC, and 0.03 ng/mL and 0.5 ng/mL for 25-OHC, respectively. In conclusion, we have validated the developed method for linearity, accuracy and precision and performed microanalysis for metabolites of cholesterol in human serum by a novel Ag+CIS method provides reproducibility and reliability for qualitative and quantitative analysis.
URI
http://pubs.kist.re.kr/handle/201004/44161
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KIST Publication > Conference Paper
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