Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds
- Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds
- Lee, Benjamin Li-Ping; 전호정; Aijun Wang; Zhiqiang Yan; Jian Yu; Grigoropoulos, Costas P.; Li, Song
- Electrospinning; Nanofibrous scaffolds; Pore size; Cell infiltration; Femtosecond laser ablation
- Issue Date
- Acta Biomaterialia
- VOL 8, NO 7, 2648-2658
- Electrospun scaffolds are used extensively in tissue-engineering applications as they offer a cell-friendly
microenvironment. However, one major limitation is the dense fibers, small pore size and consequently
poor cell infiltration. Here, we employ a femtosecond (FS) laser system to ablate and create microscale
features on electrospun poly(L-lactide) (PLLA) nanofibrous scaffolds. Upon determining the ablation
parameters, we pattern structured holes with diameters of 50, 100 and 200 μm and spacings of 50 and
200 μm between adjacent holes on the scaffolds. The elastic moduli of ablated scaffolds decrease with
the decrease in spacing and the increase in hole size. Cells seeded on the laser-ablated scaffolds exhibit
different morphology but similar proliferation rate when compared with control (non-ablated) scaffold.
Furthermore, animal studies indicate that ablated scaffolds facilitate endothelial cell ingrowth as well as
drastically increase M2 macrophage and overall cell infiltration. These findings demonstrate that FS laser
ablation can be used to increase cell infiltration into nanofibrous scaffolds. Laser ablation not only can
create desired features in micrometer length scale but also presents a new approach in the fabrication
of three-dimensional porous constructs for tissue engineering.
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