Probing the function of neuronal populations: combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging

Title
Probing the function of neuronal populations: combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
Authors
Sachiko TsudaMichelle Z.L.KeeCatarina CunhJinsook KimPing YaneLeslie M. Loewe조지어거스틴
Keywords
Optogenetics; Channelrhodopsin; Digital micromirror device; Voltage-sensitive dye imaging; Inhibitory circuitry; Cerebellum
Issue Date
2013-01
Publisher
Neuroscience research
Citation
VOL 75, NO 1, 76-81
Abstract
Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits.
URI
http://pubs.kist.re.kr/handle/201004/44373
ISSN
01680102
Appears in Collections:
KIST Publication > Article
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