Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
- Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
- Jin, Lei; Han, Zhou; Platisa, Jelena; Wooltorton, Julian R. A.); 래리코헨; Pieribone, Vincent A.
- Issue Date
- VOL 75, NO 5, 779-785
- Monitoring neuronal electrical activity using fluorescent
protein-based voltage sensors has been limited
by small response magnitudes and slow kinetics of
existing probes. Here we report the development
of a fluorescent protein voltage sensor, named
ArcLight, and derivative probes that exhibit large
changes in fluorescence intensity in response to
voltage changes. ArcLight consists of the voltagesensing
domain of Ciona intestinalis voltage-sensitive
phosphatase and super ecliptic pHluorin that
carries the point mutation A227D. The fluorescence
intensity of ArcLight A242 decreases by 35% in response
to a 100mV depolarization when measured
in HEK293 cells, which is more than five times larger
than the signals from previously reported fluorescent
protein voltage sensors. We show that the combination
of signal size and response speed of these new
probes allows the reliable detection of single action
potentials and excitatory potentials in individual
neurons and dendrites.
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