Enzyme immobilization on carbon nanomaterials: Loading density investigation and zeta potential analysis
- Enzyme immobilization on carbon nanomaterials: Loading density investigation and zeta potential analysis
- 민경선; Jungbae Kim; Kyungmoon Park; Young Je Yoo
- Enzyme immobilization; Carbon nanomaterial; High loading density; Zeta-potential analysis
- Issue Date
- Journal of molecular catalysis. B, Enzymatic
- VOL 83, 87-93
- Although enzymes are attractive catalysts due to their own specificity, industrial applications have been
hampered because of their cost, reusability, and easy deactivation under conditions far from their optimum.
Immobilization has been the most popular and successful approach to overcome these limitations
and enable enzymes to be used in large scale industrial processes. Recent progress in nanotechnology
leads research interests toward the immobilized enzyme on/into nanoscale support material. In this
study, carbon nanopowder and single-walled carbon nanotube (SWNT) were selected as the immobilization
support and non-covalent functionalization method was developed using 1-pyrene butyric acid in
order to retain the unique properties of the carbon nanomaterial. Tyrosinase, glucose oxidase, and lipase
B were immobilized on the support and showed high loading densities; 2.09 mg tyrosinase, 0.626 mg
lipase B, and 2.50 mg glucose oxidase per mg support, respectively. The isotherm curve fitted for Langmuir
model indicated that high loading densities were based on the monolayer coverage of full surface,
not multi-layer aggregation. We attempted zeta-potential analysis for determining the influential factor
affecting the loading density and consequently it was validated that the electrostatic interaction between
the enzyme and the support is the critical factor on the high loading density in not only simple adsorption
but also in covalent immobilization.
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