Motion compensation using a suctioning stabilizer for intravital microscopy

Title
Motion compensation using a suctioning stabilizer for intravital microscopy
Authors
Claudio Vinegoni이성온Rostic GorbatovRalph Weissleder
Keywords
intravital microscopy; motion compensation; optical microscopy; in vivo imaging; cardiac imaging
Issue Date
2012-12
Publisher
IntraVital
Citation
VOL 1, NO 2, 115-121
Abstract
Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution.
URI
http://pubs.kist.re.kr/handle/201004/44456
ISSN
21659079
Appears in Collections:
KIST Publication > Article
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