Endothelial differentiation and vasculogenesis induced by three-dimensional adipose-derived stem cells

Title
Endothelial differentiation and vasculogenesis induced by three-dimensional adipose-derived stem cells
Authors
박인수김수현정영미이종원김상헌
Keywords
adipose-derived stem cell; vasculogenesis; endothelial differentiation; 3D stem cells cluster; angiogenesis; hypoxic induction
Issue Date
2013-01
Publisher
The anatomical record : advances in integrative anatomy and evolutionary biology.
Citation
VOL 296, NO 1, 168-177
Abstract
Recently, an angiogenic therapy based on adipose-derived stem cells (ASCs) in an ischemic model has been reported. This study demonstrates the differentiation of human ASCs (hASCs) into endothelial cells clusters by culturing the cells in the form of three dimensional cell masses (3DCMs), which is based on the adherent activity of ASCs for a substrate. The 3DCM composed of hASCs induced hypoxic conditions and expressed angiogenic factors, such as vascular endothelial growth factor and interleukin- 8. From immunochemical staining analysis, the 3DCMs of hASCs were CD31þ, KDRþ, and CD34þ, whereas monolayer-cultured hASCs were negative for the these markers. To evaluate the ability of vasculature to form within 3DCMs, the 3DCMs were mixed in Matrigel/fibrin gel and injected into mice. Mature tubular microvessels perfused with blood were observed in the 3DCM/gel 20 days after injection, but not in the gel alone or hASC/ gel mixture. Vasculature formed in the 3DCM/gel was recognized by antibodies against human a-smooth muscle actin, KDR, CD31, and CD34, but not by antibodies against murine antigens. These results suggest that the vasculatures originated from the embedded human cells. The 3DCMs of hASCs could function as a source of vascular cells for neovascularization, and could also be co-implanted with other cell types for regenerative medicine.
URI
http://pubs.kist.re.kr/handle/201004/44500
ISSN
19328486
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE