Endothelial differentiation and vasculogenesis induced by three-dimensional adipose-derived stem cells
- Endothelial differentiation and vasculogenesis induced by three-dimensional adipose-derived stem cells
- 박인수; 김수현; 정영미; 이종원; 김상헌
- adipose-derived stem cell; vasculogenesis; endothelial differentiation; 3D stem cells cluster; angiogenesis; hypoxic induction
- Issue Date
- The anatomical record : advances in integrative anatomy and evolutionary biology.
- VOL 296, NO 1, 168-177
- Recently, an angiogenic therapy based on adipose-derived stem cells
(ASCs) in an ischemic model has been reported. This study demonstrates
the differentiation of human ASCs (hASCs) into endothelial cells clusters
by culturing the cells in the form of three dimensional cell masses
(3DCMs), which is based on the adherent activity of ASCs for a substrate.
The 3DCM composed of hASCs induced hypoxic conditions and expressed
angiogenic factors, such as vascular endothelial growth factor and interleukin-
8. From immunochemical staining analysis, the 3DCMs of hASCs were
CD31þ, KDRþ, and CD34þ, whereas monolayer-cultured hASCs were negative
for the these markers. To evaluate the ability of vasculature to form
within 3DCMs, the 3DCMs were mixed in Matrigel/fibrin gel and injected
into mice. Mature tubular microvessels perfused with blood were observed
in the 3DCM/gel 20 days after injection, but not in the gel alone or hASC/
gel mixture. Vasculature formed in the 3DCM/gel was recognized by antibodies
against human a-smooth muscle actin, KDR, CD31, and CD34, but
not by antibodies against murine antigens. These results suggest that the
vasculatures originated from the embedded human cells. The 3DCMs of
hASCs could function as a source of vascular cells for neovascularization,
and could also be co-implanted with other cell types for regenerative medicine.
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