Protective effects of a compound isolated from Alnus japonica on oxidative stress-induced death in transformed retinal ganglion cells
- Protective effects of a compound isolated from Alnus japonica on oxidative stress-induced death in transformed retinal ganglion cells
- 조형; 최순정; 정상훈
- Alnus japonica; Hirsutenone; Neuroprotection; RGC-5; Glaucoma
- Issue Date
- Food and chemical toxicology
- VOL 56, 425-435
- Here, we investigated whether hirsutenone, a compound isolated from Alnus japonica, was able to attenuate
oxidative stress-induced death in transformed retinal ganglion (RGC-5) cells.
Hirsutenone effectively protected RGC-5 cells from oxidative insult induced by, l-buthionine-(S,R)-sulfoximine
(BSO) plus glutamate in a concentration-dependent manner, as demonstrated by propidium
iodide (PI)/Hoechst 33342 double staining, flow cytometry, and MTT assays. Moreover, hirsutenone
inhibited the increase in apoptotic protein expression resulting from BSO plus glutamate.
Hirsutenone also effectively inhibited sodium nitroprusside (SNP)-induced lipid peroxidation in rat
brain homogenates. To investigate the effects of hirsutenone in vivo, we used N-methyl-D-aspartate
(NMDA) as a negative insult on the retinas of rats. NMDA affects the thinning of the inner plexiform layer
(IPL) and causes an increase in the number of terminal deoxynucleotidyl transferase-mediated dUTP
nick-end labeling (TUNEL)-positive ganglion cells in the ganglion cell layer (GCL). Hirsutenone treatment
led to a reduction in NMDA-induced IPL and TUNEL staining of the GCL.
In conclusion, hirsutenone isolated from A. japonica may act as neuroprotective agent for conditions
such as glaucoma.
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